2nd EuroFlow PID Course, Prague CZ

The EuroFlow consortium is proud to announce the 2nd EuroFlow PID Course, taking place in Prague, 31 May - 2 June, 2027.

The EuroFlow PID course is an advanced course on the application of flow cytometry in the diagnosis and classification of Primary Immunodeficiencies. It offers in-depth training on the analysis and interpretation of standardized flow cytometry (FC) data in the context of primary immunodeficiencies (PID). The program builds upon the EuroFlow IEI-PID algorithm, published by Van Dongen et al. (2019), a widely adopted tool for screening PID disorders with flow cytometry. For classifying antibody deficiencies, this algorithm recommends using IgH isotype tubes in parallel with the central PID orientation analysis, whereas for excluding (S)CID combined analysis of PIDOT and SCID/RTE tubes are advised. Such detailed level of analysis of T- and B-cell compartments contributes to early/accurate diagnosis and classification of IEI-PID patients.

To fully benefit from this program, we strongly recommend that participants have experience with flow cytometry and basic immunology.

Short presentations will detail the three relevant tubes of the PIDOT algorithm (PIDOT, SCID/RTE and IgH). Through live gating demonstrations, special attention will be given to help recognize key (maturation) patterns and defects within the blood compartment, associated with IEI-PID. In interactive sessions, participants will be challenged to analyze more complex PID patient cases (such as common variable immunodeficiency (CVID) or severe combined immunodeficiency (SCID)) with BD InfinicytTM software. These sessions will provide participants the opportunity to gain more hands-on experience with standardized EuroFlow IEI-PID gating strategy approaches and will support the identification of rare but clinically relevant immunophenotypic patterns of IEI-PID. Throughout the course, interactive quizzes will evaluate the comprehension of key concepts.

During the course, time is reserved to introduce the EuroFlow PIDOT External Quality Assessment Program. This scheme is specifically designed for laboratories that incorporated the PIDOT panel in their routine diagnostics and is organized biannually. Consisting of both a wet and dry part, it provides feedback on the quality (i.e., accuracy, reproducibility and result interpretation) of individual laboratories using the EuroFlow approach (Neirinck et al. 2024), but importantly also serves to expose the participant to rare cases.

Beyond its educational content, the course creates an environment for participants to network with leading experts and exchange insights with professionals in the field, promoting collaboration and enhancing diagnostic practices.

This is the preliminary program (version October 2026)

The 2nd EuroFlow IEI/PID Course will take place in the capital of the Czech Republic, Prague. The course is at the

Second Faculty of Medicine, Plzeňská Campus

Address: Plzeňská 311, 150 06 Praha 5-Motol, Czechia

The venue can be reached with tram 9 and 10 from the city center.
Uber and Bolt are available througout the city.
Closest hotel is Hotel Golf.

Prague’s main airport is Václav Havel Airport, PRG. From this airport to the city center, you have several transport options to reach the town center and the campus.

Credit cards are accepted.

The non-discounted price for the three-day course is € 850,-. Payment needs to be made upon registration.
NB: Employees of public and or academic laboratories (University hospitals, public hospitals and government-supported labs and research centers) get a 50% discount. Make sure to tick the box when completing your registration!

This includes:

• Materials
• Lunches and Breaks
• Networking event

Please note: You need to bring your own laptop computer. You need to be able to connect it with the local WiFi.

Registration

Registration will open November 17, 2026.

Information

For more info please contact campus@euroflow.org.