Standardized EuroFlow Protocols

Pre-analytical protocols

• EuroFlow target MFI values for Rainbow beads Version 1.10 - 16 november 2023
• EuroFlow SOP for CSF Sample Preparation and SST Staining Version 1.0 - May 2024
• EuroFlow SOP for DxFlex instrument setup and compensation Version 1.2 - 19 July 2023
• EuroFlow SOP for Sample Preparation Version 1.7 - 1 november 2023
• EuroFlow SOP for Instrument set-up and compensation for BD LSR II and BD FACS Canto Version 1.2.1 - 29 October 2019
• EuroFlow SOP for instrument set-up and compensation for BD Lyric instrument for the 8-color panels Version 1.8 - 1 July 2019
• EuroFlow SOP for Instrument set-up and compensation for BD LSRFortessa X-20 Instruments Version 2.2 - September 2024
• EuroFlow SOP for Instrument set-up and compensation for Navios Instruments Version 1.5.1 - 29 October 2019
• EuroFlow SOP for bulk lysis in MRD panels Version 1.3 - 25 June 2018
• EuroFlow guidelines for BM sample collection and transport for MM MRD measurements Version 1.0 - 3 April 2018

Analytical protocols

• Composition and technical info of EuroFlow panels for PID Version 1.2 - 14 September 2022
• Composition and technical info of EuroFlow panels for hematological malignancies Version 1.11 - 14 November 2023
• List of reference and alternative antibodies for hematol malignancies panels Version 1.13 - 13 November 2023
• Uniform coding of EuroFlow panels Version 1.1 - March 2019

Data analysis

• Analysis template BCP-ALL MRD panel (Verbeek et al., 2021)

Downloads for the BD Lyric

• Reagent files (zip) (download and extract)
• Assay files (zip) (download and extract)
• Label file (xml)

Reference values per age group

• Reference values for major populations of blood leukocytes (Euroflow PID orientation tube), version 1.1, July 2024
• Distribution major populations of blood B-cells and plasma cells childeren (EuroFlow IgH-isotypes tube), version 1.1, July 2024
• Distribution major populations of blood TCD4 cells in healthy donors grouped by age (EuroFlow IMM TCD tube), version 1.1, July 2024
• Reference values for PB leukocyte populations (EuroFlow LST tube) version 1.1, July 2024
• Reference values for the major populations of blood leukocytes (Euroflow PIDOT tube) version 1.1, October 2024
• Reference values for PB leukocyte populations (EuroFlow PIDOT tube) version 1.1, October 2024