Standardized EuroFlow Protocols

Pre-analytical protocols

• EuroFlow target MFI values for Rainbow beads. Version 1.20 - April 2025
• EuroFlow SOP for pediatric solid tumor. Version 1.4 - June 2025
• EuroFlow SOP for CSF sample preparation and SST staining. Version 1.0 - May 2024
• EuroFlow SOP for sample preparation. Version 1.7 - November 2023
• EuroFlow SOP for bulk lysis in MRD panels. Version 2.0 - June 2025
• EuroFlow SOP for instrument set-up and compensation for DxFlex. Version 2.0 - February 2025
• EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto II. Version 2.0 - February 2025
• EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 2.0 - June 2025
• EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.3 - February 2025
• EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 - October 2019
• EuroFlow guidelines for BM sample collection and transport for MM MRD measurements. Version 1.0 - April 2017

Analytical protocols

• Composition and technical info of EuroFlow panels for PID. Version 1.2 - September 2022
• Composition and technical info of EuroFlow panels for hematological malignancies. Version 1.12 - February 2025
• Composition and Technical info of EuroFlow antibody tubes for immune monitoring - February 2025
• List of reference and alternative antibodies for hematological malignancies panels. Version 1.14 - February 2025
• Uniform coding of EuroFlow panels. Version 1.1 - March 2019

Data analysis

• Analysis template BCP-ALL MRD panel (Verbeek et al., 2021)

Downloads for the BD Lyric

• Reagent files (zip) (download and extract) - June 2025
• Assay files (zip) (download and extract) - June 2025
• Label file (xml)

Reference values per age group

• Distribution major populations of blood B-cells and plasma cells in children and adult healthy donors (EuroFlow IgH-isotypes tube). Version 1.1 - July 2024
• Distribution major populations of blood TCD4 cells in healthy donors grouped by age (EuroFlow IMM TCD tube), version 1.1 - July 2024
• Reference values for PB leukocyte populations (EuroFlow LST) version 1.1 - July 2024
• Reference values for PB leukocyte populations in percentages (EuroFlow PIDOT). Version 1.2 - December 2024
• Reference values for PB leukocyte populations in absolute numbers of cells (EuroFlow PIDOT). Version 1.1 - July 2024

Reference files from healthy donors

• Normal ALOT
• Normal LST
• Normal SST
• Normal PCST
• Normal PIDOT
• Normal SCIDTRE
• Normal IgH