Publications

EuroFlow and its activities: Introduction to the special EuroFlow issue of The Journal of Immunological Methods
van Dongen JJM, O'Gorman MRG, Orfao A
Reference: J Immunol Methods 2019; 475: 112704
Immunophenotypic dissection of normal hematopoiesis
Orfao A, Matarraz S, Pérez-Andrés M, Almeida J, Teodosio C, Berkowska MA, van Dongen JJM; EuroFlow
Reference: J Immunol Methods 2019; 475: 112684
Comments on EuroFlow standard operating procedures for instrument setup and compensation for BD FACS Canto II, Navios and BD FACS Lyric instruments
Glier H, Novakova M, Te Marvelde J, Bijkerk A, Morf D, Thurner D, Rejlova K, Lange S, Finke J, van der Sluijs-Gelling A, Sedek L, Flores-Montero J, Böttcher S, Fernandez P, Ritgen M, van Dongen JJM, Orfao A, van der Velden VHJ, Kalina T
Reference: J Immunol Methods 2019; 475: 112680
EuroFlow Lymphoid Screening Tube (LST) data base for automated identification of blood lymphocyte subsets
Flores-Montero J, Grigore G, Fluxá R, Hernández J, Fernandez P, Almeida J, Muñoz N, Böttcher S, Sedek L, van der Velden V, Barrena S, Hernández A, Paiva B, Lecrevisse Q, Lima M, Santos AH, van Dongen JJM, Orfao A
Reference: J Immunol Methods 2019; 475: 112662
From big flow cytometry datasets to smart diagnostic strategies: The EuroFlow approach
Pedreira CE, Costa ESD, Lecrevise Q, Grigore G, Fluxa R, Verde J, Hernandez J, van Dongen JJM, Orfao A; EuroFlow
Reference: J Immunol Methods 2019; 475: 112631
Impact of blood storage and sample handling on quality of high dimensional flow cytometric data in multicenter clinical research
Diks AM, Bonroy C, Teodosio C, Groenland RJ, de Mooij B, de Maertelaere E, Neirynck J, Philippé J, Orfao A, van Dongen JJM, Berkowska MA
Reference: J Immunol Methods 2019; 475: 112616
Fluorochrome choices for multi-color flow cytometry
Flores-Montero J, Kalina T, Corral-Mateos A, Sanoja-Flores L, Pérez-Andrés M, Martin-Ayuso M, Sedek L, Rejlova K, Mayado A, Fernández P, van der Velden V, Bottcher S, van Dongen JJM, Orfao A
Reference: J Immunol Methods 2019; 475: 112618
Frequent issues and lessons learned from EuroFlow QA
Kalina T, Brdickova N, Glier H, Fernandez P, Bitter M, Flores-Montero J, van Dongen JJM, Orfao A
Reference: J Immunol Methods 2019; 475: 112520
Differential expression of CD73, CD86 and CD304 in normal vs. leukemic B-cell precursors and their utility as stable minimal residual disease markers in childhood B-cell precursor acute lymphoblastic leukemia
Sędek Ł, Theunissen P, Sobral da Costa E, van der Sluijs-Gelling A, Mejstrikova E, Gaipa G, Sonsala A, Twardoch M, Oliveira E, Novakova M, Buracchi C, van Dongen JJM, Orfao A, van der Velden VHJ, Szczepański T; EuroFlow Consortium
Reference: J Immunol Methods 2019; 475: 112429
How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturers
Nováková M, Glier H, Brdičková N, Vlková M, Santos AH, Lima M, Roussel M, Flores-Montero J, Szczepanski T, Böttcher S, van der Velden VHJ, Fernandez P, Mejstříková E, Burgos L, Paiva B, van Dongen JJM, Orfao A, Kalina T
Reference: J Immunol Methods 2019; 475: 112388
Selection and validation of antibody clones against IgG and IgA subclasses in switched memory B-cells and plasma cells
Blanco E, Perez-Andres M, Sanoja-Flores L, Wentink M, Pelak O, Martín-Ayuso M, Grigore G, Torres-Canizales J, López-Granados E, Kalina T, van der Burg M, Arriba-Méndez S, Santa Cruz S, Puig N, van Dongen JJM, Orfao A
Reference: J Immunol Methods 2019; 475: 112372
Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland
Glier H, Heijnen I, Hauwel M, Dirks J, Quarroz S, Lehmann T, Rovo A, Arn K, Matthes T, Hogan C, Keller P, Dudkiewicz E, Stüssi G, Fernandez P; Swiss Cytometry Society
Reference: J Immunol Methods 2019; 475: 112348
Defects in memory B-cell and plasma cell subsets expressing different immunoglobulin-subclasses in patients with CVID and immunoglobulin subclass deficiencies
Blanco E, Pérez-Andrés M, Arriba-Méndez S, Serrano C, Criado I, Del Pino-Molina L, Silva S, Madruga I, Bakardjieva M, Martins C, Serra-Caetano A, Romero A, Contreras-Sanfeliciano T, Bonroy C, Sala F, Martín A, Bastida JM, Lorente F, Prieto C, Dávila I, Marcos M, Kalina T, Vlkova M, Chovancova Z, Cordeiro AI, Philippé J, Haerynck F, López-Granados E, Sousa AE, van der Burg M, van Dongen JJM, Orfao A; EuroFlow PID group
Reference: J Allergy Clin Immunol 2019; 144: 809-824
EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System
van Dongen JJM, van der Burg M, Kalina T, Perez-Andres M, Mejstrikova E, Vlkova M, Lopez-Granados E, Wentink M, Kienzler AK, Philippé J, Sousa AE, van Zelm MC, Blanco E, Orfao A
Reference: Front Immunol 2019; 10: 1271
The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System
van der Burg M, Kalina T, Perez-Andres M, Vlkova M, Lopez-Granados E, Blanco E, Bonroy C, Sousa AE, Kienzler AK, Wentink M, Mejstríková E, Šinkorova V, Stuchly J, van Zelm MC, Orfao A, van Dongen JJM
Reference: Front Immunol 2019; 10: 246
Residual normal B-cell profiles in monoclonal B-cell lymphocytosis versus chronic lymphocytic leukemia
Criado I, Blanco E, Rodríguez-Caballero A, Alcoceba M, Contreras T, Gutiérrez ML, Romero A, Fernández-Navarro P, González M, Solano F, Gómez C, Pérez-Andrés M, van Dongen JJM, Almeida J, Orfao A; EuroFlow PID Group and The Primary Health Care Group of Salamanca for the Study of MBL
Reference: Leukemia 2018; 32: 2701-2705
Next generation flow for minimally-invasive blood characterization of MGUS and multiple myeloma at diagnosis based on circulating tumor plasma cells (CTPC)
Sanoja-Flores L, Flores-Montero J, Garcés JJ, Paiva B, Puig N, García-Mateo A, García-Sánchez O, Corral-Mateos A, Burgos L, Blanco E, Hernández-Martín J, Pontes R, Díez-Campelo M, Millacoy P, Rodríguez-Otero P, Prosper F, Merino J, Vidriales MB, García-Sanz R, Romero A, Palomera L, Ríos-Tamayo R, Pérez-Andrés M, Blanco JF, González M, van Dongen JJM, Durie B, Mateos MV, San-Miguel J, Orfao A; EuroFlow consortium
Reference: Blood Cancer J 2018; 8: 117
Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood
Blanco E, Pérez-Andrés M, Arriba-Méndez S, Contreras-Sanfeliciano T, Criado I, Pelak O, Serra-Caetano A, Romero A, Puig N, Remesal A, Torres Canizales J, López-Granados E, Kalina T, Sousa AE, van Zelm M, van der Burg M, van Dongen JJM, Orfao A; EuroFlow PID group
Reference: J Allergy Clin Immunol 2018; 141: 2208-2219.e16
Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia
Lhermitte L, Mejstrikova E, van der Sluijs-Gelling AJ, Grigore GE, Sedek L, Bras AE, Gaipa G, Sobral da Costa E, Novakova M, Sonneveld E, Buracchi C, de Sá Bacelar T, Te Marvelde JG, Trinquand A, Asnafi V, Szczepanski T, Matarraz S, Lopez A, Vidriales B, Bulsa J, Hrusak O, Kalina T, Lecrevisse Q, Martin Ayuso M, Brüggemann M, Verde J, Fernandez P, Burgos L, Paiva B, Pedreira CE, van Dongen JJM, Orfao A, van der Velden VHJ
Reference: Leukemia 2018; 32: 874-881
Detailed immunophenotyping of B-cell precursors in regenerating bone marrow of acute lymphoblastic leukaemia patients: implications for minimal residual disease detection
Theunissen PMJ, Sedek L, De Haas V, Szczepanski T, Van Der Sluijs A, Mejstrikova E, Nováková M, Kalina T, Lecrevisse Q, Orfao A, Lankester AC, van Dongen JJM, Van Der Velden VHJ on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)
Reference: British Journal of Haematology 2017; 178: 257-266
Lot-to-lot stability of antibody reagents for flow cytometry
Böttcher S, van der Velden VHJ, Villamor N, Ritgen M, Flores-Montero J, Murua Escobar H, Kalina T, Brüggemann M, Grigore G, Martin-Ayuso M, Lecrevisse Q, Pedreira CE, van Dongen JJM, Orfao A
Reference: Journal of Immunological Methods 2017; doi: 10.1016/j.jim.2017.03.018
Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples
van der Velden VH, Flores-Montero J, Perez-Andres M, Martin-Ayuso M, Crespo O, Blanco E, Kalina T, Philippé J, Bonroy C, de Bie M, Te Marvelde J, Teodosio C, Corral Mateos A, Kanderová V, van der Burg M, Van Hoof D, van Dongen JJ, Orfao A
Reference: Journal of Immunological Methods 2017; doi: 10.1016/j.jim.2017.03.011
Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma
Flores-Montero J, Sanoja-Flores L, Paiva B, Puig N, García-Sánchez O, Böttcher S, van der Velden VH, Pérez-Morán JJ, Vidriales MB, García-Sanz R, Jimenez C, González M, Martínez-López J, Corral-Mateos A, Grigore GE, Fluxá R, Pontes R, Caetano J, Sedek L, Del Cañizo MC, Bladé J, Lahuerta JJ, Aguilar C, Bárez A, García-Mateo A, Labrador J, Leoz P, Aguilera-Sanz C, San-Miguel J, Mateos MV, Durie B, van Dongen JJ, Orfao A
Reference: Leukemia 2017; doi: 10.1038/leu.2017.29
Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia
Theunissen P, Mejstrikova E, Sedek L, van der Sluijs-Gelling AJ, Gaipa G, Bartels M, Sobral da Costa E, Kotrová M, Novakova M, Sonneveld E, Buracchi C, Bonaccorso P, Oliveira E, Te Marvelde JG, Szczepanski T, Lhermitte L, Hrusak O, Lecrevisse Q, Grigore GE, Fronková E, Trka J, Brüggemann M, Orfao A, van Dongen JJ, van der Velden VH on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)
Reference: Blood 2017; 129: 347-357
Utility of CD54, CD229, and CD319 for the identification of plasma cells in patients with clonal plasma cell diseases
Pojero F, Flores-Montero J, Sanoja L, Pérez JJ, Puig N, Paiva B, Bottcher S, van Dongen JJ, Orfao A on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)
Reference: Cytometry Part B: Clinical Cytometry 2016; 90: 91-100
Quality Assessment Program for EuroFlow Protocols: Summary results of four-year (2010 - 2013) quality assurance rounds
Tomas Kalina, Juan Flores-Montero, Quentin Lecrevisse, Carlos E. Pedreira, Vincent H.J. van der Velden, Michaela Novakova, Ester Mejstrikova, Ondrej Hrusak, Sebastian Böttcher, Dennis Karsch, Lukasz Sedek, Amelie Trinquand, Nancy Boeckx, Joana Caetano, Vahid Asnafi, Paulo Lucio, Margarida Lima, Ana Helena Santos, Paola Bonaccorso, Alita J. van der Sluijs-Gelling, Anton W. Langerak, Marta Martin-Ayuso, Tomasz Szczepanski, Jacques J.M. van Dongen, Alberto Orfao
Reference: Cytometry Part A 2015; 87A: 145-156
Overview of clinical flow cytometry data analysis: recent advances and future challenges
Carlos E. Pedreira, Elaine S. Costa, Quentin Lecrevisse, Jacques J.M. van Dongen, Alberto Orfao, on behalf of the EuroFlow Consortium
Reference: Trends in Biotechnology 2013; 31: 415-425
EuroFlow: Resetting leukemia and lymphoma immunophenotyping. Basis for companion diagnostics and personalized medicine
JJM van Dongen and A Orfao
Reference: Leukemia 2012; 26: 1899-1907
EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes
JJM van Dongen, L Lhermitte, S Böttcher, J Almeida, VHJ van der Velden, J Flores Montero, A Rawstron, V Asnafi, Q Lécrevisse, P Lucio, E Mejstrikova, T Szczepanski, T Kalina, R de Tute, M Brüggemann, L Sedek, M Cullen, AW Langerak, A Mendonça, E Macintyre, M Martin-Ayuso, O Hrusak, MB Vidriales and A Orfao on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)
Reference: Leukemia 2012; 26: 1908-1975
Flow cytometric immunobead assay for fast and easy detection of PML–RARA fusion proteins for the diagnosis of acute promyelocytic leukemia
EHA Dekking, VHJ van der Velden, R Varro, H Wai, S Böttcher, M Kneba, E Sonneveld, A Koning, N Boeckx, N Van Poecke, P Lucio, A Mendonca, L Sedek, T Szczepanski, T Kalina, V Kanderová, P Hoogeveen, J Flores-Montero, MC Chillón, A Orfao, J Almeida, P Evans, M Cullen, AL Noordijk, PM Vermeulen, MT de Man, EP Dixon, WM Comans-Bitter and JJM van Dongen on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Reference: Leukemia 2012; 26: 1976-1985
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
T Kalina, J Flores-Montero, VHJ van der Velden, M Martin-Ayuso, S Böttcher, M Ritgen, J Almeida, L Lhermitte, V Asnafi, A Mendonça, R de Tute, M Cullen, L Sedek, MB Vidriales, JJ Pérez, JG te Marvelde, E Mejstrikova, O Hrusak, T Szczepanski, JJM van Dongen and A Orfao on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Reference: Leukemia 2012; 26: 1986-2010
Harmonization of Light Scatter and Fluorescence Flow Cytometry Profiles Obtained After Staining Peripheral Blood Leucocytes for Cell Surface-Only Versus Intracellular Antigens with the Fix & Perm™ Reagent
Elaine Sobral da Costa, Rodrigo Tosta Peres, Julia Almeida, Quentin Lécrevisse, María Elena Arroyo, Cristina Teodósio, Carlos Eduardo Pedreira, Jacques J. M. van Dongen, and Alberto Orfao on behalf of the EuroFlow Consortium
Reference: Cytometry Part B (Clinical Cytometry) 2010; 78B: 11-20
Automated pattern-guided principal component analysis vs expert-based immunophenotypic classification of B-cell chronic lymphoproliferative disorders: a step forward in the standardization of clinical immunophenotyping
ES Costa, CE Pedreira, S Barrena, Q Lecrevisse, J Flores, S Quijano, J Almeida, M Del Carmen Garcia-Macias, S Böttcher, JJM van Dongen, A Orfao
Reference: Leukemia 2010; 24: 1927-1933
Detection of fusion genes at the protein level in leukemia patients via the flow cytometric immunobead assay
E. Dekking, V.H.J, van der Velden, S. Böttcher, M. Brüggemann, E. Sonneveld, A. Koning-Goedheer, N. Boeckx, P. Lucio, L. Sedek, T. Szczepanski, T. Kalina, M. Kovac, P. Evans, P.G. Hoogeveen, J. Flores-Montero, A. Orfao, W.M. Comans-Bitter, F.J.T. Staal, J.J.M. van Dongen on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Reference: Best Practice & Research Clinical Haematology 2010; 23: 333-345
Flow cytometric immunobead assay for the detection of BCR–ABL fusion proteins in leukemia patients
F Weerkamp, E Dekking, YY Ng, VHJ van der Velden, H Wai, S Böttcher, M Brüggemann, AJ van der Sluijs, A Koning, N Boeckx, N Van Poecke, P Lucio, A Mendonça, L Sedek, T Szczepanski, T Kalina, M Kovac, PG Hoogeveen, J Flores-Montero, A Orfao, E Macintyre, L Lhermitte, R Chen, KAJ Brouwer-De Cock, A van der Linden, ALNoordijk, WM Comans-Bitter, FJT Staal and JJM van Dongen On behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Reference: Leukemia 2009; 23: 1106-1117
A Probabilistic Approach for the Evaluation of Minimal Residual Disease by Multiparameter Flow Cytometry in Leukemic B-Cell Chronic Lymphoproliferative Disorders
C.E. Pedreira, E.S. Costa, J. Almeida, C. Fernandez, S. Quijano, J. Flores, S. Barrena, Q. Lecrevisse, J.J.M. Van Dongen, A. Orfao, on behalf of the EuroFlow Consortium
Reference: Cytometry Part A. 2008; 73A: 1141-1150
Generation of Flow Cytometry Data Files with a Potentially Infinite Number of Dimensions
Carlos E. Pedreira, Elaine S. Costa, Susana Barrena, Quentin Lecrevisse, Julia Almeida, Jacques J. M. van Dongen, Alberto Orfao; on behalf of EuroFlow Consortium
Reference: Cytometry Part A. 2008; 73A: 834-846
EDITORIAL: Infinite multidimensionality
Attila Tárnok
Reference: Cytometry Part A. 2008; 73A: 777-778