Publications

Phenotypic profiling of CD34+ cells by advanced flow cytometry improves diagnosis of juvenile myelomonocytic leukemia

Bugarin C, Antolini L, Buracchi C, Matarraz S, Coliva TA, van der Velden VH, Szczepanski T, Sobral da Costa E, van der Sluijs A, Novakova M, Mejstrikova E, Nierkens A, Vieira de Mello F, Fernandez P, Aanei C, Sędek L, Strocchio L,Masetti R, Sainati L, Philippé J, Valsecchi MG, Locatelli F, van Dongen JJM, Biondi A, Orfao A, Gaipa G

Reference: Haaematologica, Vol. 109 No. 2 (2024): February, 2024

Link: https://haematologica.org/article/view/haematol.2023.282805

Automated EuroFlow approach for standardized in-depth dissection of human circulating B-cells and plasma cells

Delgado AH, Fluxa R, Perez-Andres M, Diks AM, van Gaans-van den Brink JAM, Barkoff AM, Blanco E, Torres-Valle A, Berkowska MA Grigor G, van Dongen JJM, and Orfao A on behalf of The PERISCOPE Consortium

Reference: Frontiers in Immunology Vol 14 - 2023

Link: https://doi.org/10.3389/fimmu.2023.1268686

Minimal residual disease assessment in B-cell precursor acute lymphoblastic leukemia by semi-automated identification of normal hematopoietic cells: A EuroFlow study.

80. Verbeek MWC, Rodríguez BS, Sedek L, Laqua A, Buracchi C, Buysse M, Reiterová M, Oliveira E, Morf D, Oude Alink SR, Barrena S, Kohlscheen S, Nierkens S, Hofmans M, Fernandez P, de Costa ES, Mejstrikova E, Szczepanski T, Slota L, Brüggemann M, Gaipa G, Grigore G, van Dongen JJM, Orfao A, van der Velden VHJ

Reference: Cytometry B Clin Cytom. 2023 Sep 22. Doi: 10.1002/cyto.b.22143

Link: https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22143

Technical alignment of standardized flow cytometry procedures among Cutaneous Lymphoma Working Groups (CLWGs) and EuroFlow centers: update on a Multicenter Study

De Bie F, van der Sluijs-Gelling A, Najidh S, de Haan S,Flores-Montero J, Orfao A, Dongen JJM, AlmeidaJ, and Vermeer M

Reference: European Journal of Cancer - Oral presentations: Clinical studies 1| Vol. 190, Suppl. 1, 113000, September 2023

Link: https://doi.org/10.1016/j.ejca.2023.113000

Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations

van der Pan K, Khatri I, de Jager AL, Louis A, Kassem S, Naber BAE, de Laat IF, Hameetman M, Comans SET, Orfao A, van Dongen JJM, Díez P, Teodosio C

Reference: Reference: Front Immunol 2023; 14: 1191992

Link: https://pubmed.ncbi.nlm.nih.gov/37275858/

Development of a standardized and validated flow cytometry approach for monitoring of innate myeloid immune cells in human blood

van der Pan K, de Bruin-Versteeg S, Damasceno D, Hernández-Delgado A, van der Sluijs-Gelling AJ, van den Bossche WBL, de Laat IF, Díez P, Naber BAE, Diks AM, Berkowska MA, de Mooij B, Groenland RJ, de Bie FJ, Khatri I, Kassem S, de Jager AL, Louis A, Almeida J, van Gaans-van den Brink JAM, Barkoff AM, He Q, Ferwerda G, Versteegen P, Berbers GAM, Orfao A, van Dongen JJM, Teodosio C

Reference: Front Immunol 2022; 13: 935879

Link: https://pubmed.ncbi.nlm.nih.gov/36189252/

The EuroFlow PID Orientation Tube in the diagnostic workup of primary immunodeficiency: Daily practice performance in a tertiary university hospital

Neirinck J, Emmaneel A, Buysse M, Philippé J, Van Gassen S, Saeys Y, Bossuyt X, De Buyser S, van der Burg M, Pérez-Andrés M, Orfao A, van Dongen JJM, Lambrecht BN, Kerre T, Hofmans M, Haerynck F, Bonroy C.

Reference: Front Immunol 2022 Sep 13;13:

Link: https://pubmed.ncbi.nlm.nih.gov/36177024/

Bone Marrow Stromal Cell Regeneration Profile in Treated B-Cell Precursor Acute Lymphoblastic Leukemia Patients: Association with MRD Status and Patient Outcome

Oliveira E, Costa ES, Ciudad J, Gaipa G, Sedek Ł, Barrena S, Szczepanski T, Buracchi C, Silvestri D, Siqueira PFR, Mello FV, Torres RC, Oliveira LMR, Fay-Neves IVC, Sonneveld E, van der Velden VHJ, Mejstrikova E, Ribera JM, Conter V, Schrappe M, van Dongen JJM, Land MGP, Orfao A; EuroFlow Consortium

Reference: Cancers (Basel) 2022 Jun 23;14

Link: https://pubmed.ncbi.nlm.nih.gov/35804860/

Quality Assessment of a Large Multi-Center Flow Cytometric Dataset of Acute Myeloid Leukemia Patients-A EuroFlow Study

Bras AE, Matarraz S, Nierkens S, Fernández P, Philippé J, Aanei CM, de Mello FV, Burgos L, van der Sluijs-Gelling AJ, Grigore GE, van Dongen JJM, Orfao A, van der Velden VHJ, On Behalf Of The EuroFlow Consortium

Reference: Cancers (Basel) 2022 Apr 15;14

Link: https://pubmed.ncbi.nlm.nih.gov/35454917/

Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study

Brouwer N, Matarraz S, Nierkens S, Hofmans M, Nováková M, da Costa ES, Fernandez P, Bras AE, de Mello FV, Mejstrikova E, Philippé J, Grigore GE, Pedreira CE, van Dongen JJM, Orfao A, van der Velden VHJ, On Behalf Of The EuroFlow Consortium.

Reference: Cancers (Basel) 2022 Mar 21;14

Link: https://pubmed.ncbi.nlm.nih.gov/35326734/

High-Sensitive TRBC1-Based Flow Cytometric Assessment of T-Cell Clonality in Tαβ-Large Granular Lymphocytic Leukemia

Muñoz-García N, Morán-Plata FJ, Villamor N, Lima M, Barrena S, Mateos S, Caldas C, van Dongen JJM, Orfao A, Almeida J.

Reference: Cancers (Basel) 2022 Jan 14;14

Link: https://pubmed.ncbi.nlm.nih.gov/35053571/

Impact of Pre-Analytical and Analytical Variables Associated with Sample Preparation on Flow Cytometric Stainings Obtained with EuroFlow Panels

Sedek L, Flores-Montero J, van der Sluijs A, Kulis J, te Marvelde J, Philippé J, Böttcher S, Bitter WM, Caetano J, van der Velden VHJ, Sonneveld E, Buracchi C, Santos AH, Lima M, Szczepanski T, van Dongen JJM and Orfao A

Reference: Cancers 2022; 14: 473

Link: https://pubmed.ncbi.nlm.nih.gov/35158741/

Flow cytometric minimal residual disease assessment in B-cell precursor acute lymphoblastic leukaemia patients treated with CD19-targeted therapies - a EuroFlow study

Verbeek MWC, Buracchi C, Laqua A, Nierkens S, Sedek L, Flores-Montero J, Hofmans M, Sobral de Costa E, Nováková M, Mejstrikova E, Barrena S, Kohlscheen S, Szczepanowski M, Kulis J, Oliveira E, Jugooa R, de Jong AX, Szczepanski T, Philippé J, van Dongen JJM, Orfao A, Brüggemann M, Gaipa G, van der Velden VHJ

Reference: Br J Haematol 2022; 197: 76-81

Link: https://pubmed.ncbi.nlm.nih.gov/34881427/

Expert-independent classification of mature B-cell neoplasms using standardized flow cytometry: a multicentric study

Böttcher S, Engelmann R, Grigore G, Fernandez PC, Caetano J, Flores-Montero J, van der Velden VHJ, Novakova M, Philippé J, Ritgen M, Burgos L, Lecrevisse QA, Lange S, Kalina T, Verde J, Fluxa R, van Dongen JJM, Pedreira CE, Orfao A

Reference: Blood Adv 2022; 6: 976-992

Link: https://pubmed.ncbi.nlm.nih.gov/34814179/

Improved Sézary cell detection and novel insights into immunophenotypic and molecular heterogeneity in Sézary syndrome

Najidh S, Tensen CP, van der Sluijs-Gelling AJ, Teodosio C, Cats D, Mei H, Kuipers TB, Out-Luijting JJ, Zoutman WH, van Hall T, Orfao A, Almeida J, van Dongen JJM, Vermeer MH

Reference: Blood 2021; 138: 2539-2554

Link: https://pubmed.ncbi.nlm.nih.gov/34314480/

Anti-TRBC1 antibody-based flow cytometric detection of T-cell clonality: Standardization of sample preparation and diagnostic implementation

Muñoz-García N, Lima M, Villamor N, Morán-Plata FJ, Barrena S, Mateos S, Caldas C, Balanzategui A, Alcoceba M, Domínguez A, Gómez F, Langerak AW, van Dongen JJM, Orfao A, Almeida J

Reference: Cancers 2021; 13: 4379

Link: https://pubmed.ncbi.nlm.nih.gov/34503189/

Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia

Bras AE, Osmani Z, de Haas V, Jongen-Lavrencic M, te Marvelde JG, Zwaan CM, Mejstrikova E, Fernandez P, Szczepanski T, Orfao A, van Dongen JJM, van der Velden VHJ

Reference: Brit J Haematol 2021; 193: 922-27

Link: https://pubmed.ncbi.nlm.nih.gov/33161592/

B-cell regeneration profile and minimal residual disease status in bone marrow of treated multiple myeloma patients

Mendonca de Pontes R, Flores-Montero J, Sanoja-Flores L, Puig N, Pessoa de Magalhães RJ, Corral-Mateos A, Salgado AB, García-Sánchez O, Pérez-Morán J, Mateos M-V, Burgos L, Paiva B, te Marvelde J, van der Velden VHJ, Aguilar, Bárez A, García-Mateo A, Labrador J, Leoz P, Aguilera-Sanz, Durie B, van Dongen JJM, Maiolino A, Sobral da Costa E, Orfao A

Reference: Cancers 2021; 13: 1704

Link: https://pubmed.ncbi.nlm.nih.gov/33916787/

Automated identification of leukocyte subsets improves standardization of database-guided expert-supervised diagnostic orientation in acute leukemia: a EuroFlow study

Lhermitte L, Barreau S, Morf D, Fernandez P, Grigore G, Barrena S, de Bie M, Flores-Montero J, Brüggemann M, Mejstrikova E, Nierkens S, Burgos L, Caetano J, Gaipa G, Buracchi C, Sobral da Costa E, Sedek L, Szczepanski T, Aanei C-M, van der Sluijs-Gelling A, Hernández Delgado A, Fluxa R, Lecrevisse Q, Pedreira CE, van Dongen JJM, Orfao A, van der Velden VHJ, EuroFlow Consortium

Reference: Modern Pathol 2021; 34: 59-69

Link: https://pubmed.ncbi.nlm.nih.gov/32999413/

Dissection of the pre-germinal center B-cell maturation pathway in common variable immunodeficiency based on standardized flow cytometric EuroFlow tools

Del Pino-Molina L, López-Granados E, Lecrevisse Q, Torres Canizales J, Pérez-Andrés M, Blanco E, Wentink M, Bonroy C, Nechvatalova J, Milota T, Kienzler A-K, Philippé J, Sousa AE, van der Burg M, Kalina T, van Dongen JJM, Orfao A, on behalf of the EuroFlow PID Consortium

Reference: Front Immunol 2021; 11: 603972

Link: https://pubmed.ncbi.nlm.nih.gov/33679693/

EuroFlow Standardized Approach to Diagnostic Immunopheneotyping of Severe PID in Newborns and Young Children

Kalina T, Bakardjieva M, Blom M, Perez-Andres M, Barendregt B, Kanderová V, Bonroy C, Philippé J, Blanco E, Pico-Knijnenburg I, Paping JHMP, Wolska-Kuśnierz B, Pac M, Tkazcyk J, Haerynck F, Akar HH, Formánková R, Freiberger T, Svatoň M, Šedivá A, Arriba-Méndez S, Orfao A, van Dongen JJM, van der Burg M

Reference: Front Immunol 2020; 11: 371

Link: https://pubmed.ncbi.nlm.nih.gov/32265901/

Improved standardization of flow cytometric diagnostic screening of primary immunodeficiency by software-based automated gating

Linskens E, Diks AM, Neirinck J, Perez-Andres M, de Maertelaere E, Berkowska MA, Kerre T, Hofmans M, Orfao A, van Dongen JJM, Haerynck F, Philippé J, Bonroy C

Reference: Front Immunol 2020; 11:584646

Link: https://pubmed.ncbi.nlm.nih.gov/33224147/

Age distribution of multiple functionally relevant subsets of CD4+ T cells in human blood using a standardized and validated 14-color EuroFlow immune monitoring tube

Botafogo V, Pérez-Andres M, Jara-Acevedo M, Bárcena P, Grigore G, Hernández-Delgado A, Damasceno D, Comans S, Blanco E, Romero A, Arriba-Méndez S, Gastaca-Abasolo I, Pedreira CE, van Gaans-van den Brink J, Corbiere V, Mascart F, van Els CACM, Barkoff A-M, Mayado A, van Dongen JJM, Almeida J, Orfao A, on behalf of the EuroFlow and PERISCOPE consortia

Reference: Front Immunol 2020; 11: 166

Link: https://pubmed.ncbi.nlm.nih.gov/32174910/

Blood monitoring of circulating tumor plasma cells by next generation flow in multiple myeloma after therapy

Sanoja-Flores L, Flores Montero J, Puig N, Contreras-Sanfeliciano T, Pontes R, Corral-Mateos A, Garía-Sánchez O, Díez-Campelo M, Pessoa de Magalhães RJ, García-Martín L, Alonso-Alonso JM, Carcía-Mateo A, Aguilar-Franco C, Labrador J, Barez-García A, Maiolino A, Paiva B, San Miguel J, Sobral da Costa E, González M, Mateos MV, Durie B, van Dongen JJM, Orfao A, on behalf of the EuroFlow Consortium

Reference: Blood 2019; 134: 2218-22

Link: https://pubmed.ncbi.nlm.nih.gov/31697808/

Delineating Human B Cell Precursor Development With Genetically Identified PID Cases as a Model

Wentink MWJ, Kalina T, Perez-Andres M, Del Pino Molina L, IJspeert H, Kavelaars FG, Lankester AC, Lecrevisse Q, van Dongen JJM, Orfao A, van der Burg M

Reference: Front Immunol 2019; 10: 2680

Link: https://pubmed.ncbi.nlm.nih.gov/31849931/

Complete Multilineage CD4 Expression Defect Associated With Warts Due to an Inherited Homozygous CD4 Gene Mutation

Fernandes RA, Perez-Andres M, Blanco E, Jara-Acevedo M, Criado I, Almeida J, Botafogo V, Coutinho I, Paiva A, van Dongen JJM, Orfao A, Faria E

Reference: Front Immunol 2019; 10: 2502

Link: https://pubmed.ncbi.nlm.nih.gov/31781092/

EuroFlow and its activities: Introduction to the special EuroFlow issue of The Journal of Immunological Methods

van Dongen JJM, O'Gorman MRG, Orfao A

Reference: J Immunol Methods 2019; 475: 112704

Link: https://www.ncbi.nlm.nih.gov/pubmed/31758969

Immunophenotypic dissection of normal hematopoiesis

Orfao A, Matarraz S, Pérez-Andrés M, Almeida J, Teodosio C, Berkowska MA, van Dongen JJM; EuroFlow

Reference: J Immunol Methods 2019; 475: 112684

Link: https://www.ncbi.nlm.nih.gov/pubmed/31676343

Comments on EuroFlow standard operating procedures for instrument setup and compensation for BD FACS Canto II, Navios and BD FACS Lyric instruments

Glier H, Novakova M, Te Marvelde J, Bijkerk A, Morf D, Thurner D, Rejlova K, Lange S, Finke J, van der Sluijs-Gelling A, Sedek L, Flores-Montero J, Böttcher S, Fernandez P, Ritgen M, van Dongen JJM, Orfao A, van der Velden VHJ, Kalina T

Reference: J Immunol Methods 2019; 475: 112680

Link: https://www.ncbi.nlm.nih.gov/pubmed/31655051

EuroFlow Lymphoid Screening Tube (LST) data base for automated identification of blood lymphocyte subsets

Flores-Montero J, Grigore G, Fluxá R, Hernández J, Fernandez P, Almeida J, Muñoz N, Böttcher S, Sedek L, van der Velden V, Barrena S, Hernández A, Paiva B, Lecrevisse Q, Lima M, Santos AH, van Dongen JJM, Orfao A

Reference: J Immunol Methods 2019; 475: 112662

Link: https://www.ncbi.nlm.nih.gov/pubmed/31454495

From big flow cytometry datasets to smart diagnostic strategies: The EuroFlow approach

Pedreira CE, Costa ESD, Lecrevise Q, Grigore G, Fluxa R, Verde J, Hernandez J, van Dongen JJM, Orfao A; EuroFlow

Reference: J Immunol Methods 2019; 475: 112631

Link: https://www.ncbi.nlm.nih.gov/pubmed/31306640

Impact of blood storage and sample handling on quality of high dimensional flow cytometric data in multicenter clinical research

Diks AM, Bonroy C, Teodosio C, Groenland RJ, de Mooij B, de Maertelaere E, Neirynck J, Philippé J, Orfao A, van Dongen JJM, Berkowska MA

Reference: J Immunol Methods 2019; 475: 112616

Link: https://www.ncbi.nlm.nih.gov/pubmed/31181213

Fluorochrome choices for multi-color flow cytometry

Flores-Montero J, Kalina T, Corral-Mateos A, Sanoja-Flores L, Pérez-Andrés M, Martin-Ayuso M, Sedek L, Rejlova K, Mayado A, Fernández P, van der Velden V, Bottcher S, van Dongen JJM, Orfao A

Reference: J Immunol Methods 2019; 475: 112618

Link: https://www.ncbi.nlm.nih.gov/pubmed/31181212

Frequent issues and lessons learned from EuroFlow QA

Kalina T, Brdickova N, Glier H, Fernandez P, Bitter M, Flores-Montero J, van Dongen JJM, Orfao A

Reference: J Immunol Methods 2019; 475: 112520

Link: https://www.ncbi.nlm.nih.gov/pubmed/30237053

Differential expression of CD73, CD86 and CD304 in normal vs. leukemic B-cell precursors and their utility as stable minimal residual disease markers in childhood B-cell precursor acute lymphoblastic leukemia

Sędek Ł, Theunissen P, Sobral da Costa E, van der Sluijs-Gelling A, Mejstrikova E, Gaipa G, Sonsala A, Twardoch M, Oliveira E, Novakova M, Buracchi C, van Dongen JJM, Orfao A, van der Velden VHJ, Szczepański T; EuroFlow Consortium

Reference: J Immunol Methods 2019; 475: 112429

Link: https://www.ncbi.nlm.nih.gov/pubmed/29530508

How to make usage of the standardized EuroFlow 8-color protocols possible for instruments of different manufacturers

Nováková M, Glier H, Brdičková N, Vlková M, Santos AH, Lima M, Roussel M, Flores-Montero J, Szczepanski T, Böttcher S, van der Velden VHJ, Fernandez P, Mejstříková E, Burgos L, Paiva B, van Dongen JJM, Orfao A, Kalina T

Reference: J Immunol Methods 2019; 475: 112388

Link: https://www.ncbi.nlm.nih.gov/pubmed/29154914

Selection and validation of antibody clones against IgG and IgA subclasses in switched memory B-cells and plasma cells

Blanco E, Perez-Andres M, Sanoja-Flores L, Wentink M, Pelak O, Martín-Ayuso M, Grigore G, Torres-Canizales J, López-Granados E, Kalina T, van der Burg M, Arriba-Méndez S, Santa Cruz S, Puig N, van Dongen JJM, Orfao A

Reference: J Immunol Methods 2019; 475: 112372

Link: https://www.ncbi.nlm.nih.gov/pubmed/28965920

Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland

Glier H, Heijnen I, Hauwel M, Dirks J, Quarroz S, Lehmann T, Rovo A, Arn K, Matthes T, Hogan C, Keller P, Dudkiewicz E, Stüssi G, Fernandez P; Swiss Cytometry Society

Reference: J Immunol Methods 2019; 475: 112348

Link: https://www.ncbi.nlm.nih.gov/pubmed/28760670

Defects in memory B-cell and plasma cell subsets expressing different immunoglobulin-subclasses in patients with CVID and immunoglobulin subclass deficiencies

Blanco E, Pérez-Andrés M, Arriba-Méndez S, Serrano C, Criado I, Del Pino-Molina L, Silva S, Madruga I, Bakardjieva M, Martins C, Serra-Caetano A, Romero A, Contreras-Sanfeliciano T, Bonroy C, Sala F, Martín A, Bastida JM, Lorente F, Prieto C, Dávila I, Marcos M, Kalina T, Vlkova M, Chovancova Z, Cordeiro AI, Philippé J, Haerynck F, López-Granados E, Sousa AE, van der Burg M, van Dongen JJM, Orfao A; EuroFlow PID group

Reference: J Allergy Clin Immunol 2019; 144: 809-824

Link: https://www.ncbi.nlm.nih.gov/pubmed/30826363

EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System

van Dongen JJM, van der Burg M, Kalina T, Perez-Andres M, Mejstrikova E, Vlkova M, Lopez-Granados E, Wentink M, Kienzler AK, Philippé J, Sousa AE, van Zelm MC, Blanco E, Orfao A

Reference: Front Immunol 2019; 10: 1271

Link: https://www.ncbi.nlm.nih.gov/pubmed/31263462

The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System

van der Burg M, Kalina T, Perez-Andres M, Vlkova M, Lopez-Granados E, Blanco E, Bonroy C, Sousa AE, Kienzler AK, Wentink M, Mejstríková E, Šinkorova V, Stuchly J, van Zelm MC, Orfao A, van Dongen JJM

Reference: Front Immunol 2019; 10: 246

Link: https://www.ncbi.nlm.nih.gov/pubmed/30886612

Residual normal B-cell profiles in monoclonal B-cell lymphocytosis versus chronic lymphocytic leukemia

Criado I, Blanco E, Rodríguez-Caballero A, Alcoceba M, Contreras T, Gutiérrez ML, Romero A, Fernández-Navarro P, González M, Solano F, Gómez C, Pérez-Andrés M, van Dongen JJM, Almeida J, Orfao A; EuroFlow PID Group and The Primary Health Care Group of Salamanca for the Study of MBL

Reference: Leukemia 2018; 32: 2701-2705

Link: https://www.ncbi.nlm.nih.gov/pubmed/29930299

Next generation flow for minimally-invasive blood characterization of MGUS and multiple myeloma at diagnosis based on circulating tumor plasma cells (CTPC)

Sanoja-Flores L, Flores-Montero J, Garcés JJ, Paiva B, Puig N, García-Mateo A, García-Sánchez O, Corral-Mateos A, Burgos L, Blanco E, Hernández-Martín J, Pontes R, Díez-Campelo M, Millacoy P, Rodríguez-Otero P, Prosper F, Merino J, Vidriales MB, García-Sanz R, Romero A, Palomera L, Ríos-Tamayo R, Pérez-Andrés M, Blanco JF, González M, van Dongen JJM, Durie B, Mateos MV, San-Miguel J, Orfao A; EuroFlow consortium

Reference: Blood Cancer J 2018; 8: 117

Link: https://www.ncbi.nlm.nih.gov/pubmed/30455467

Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood

Blanco E, Pérez-Andrés M, Arriba-Méndez S, Contreras-Sanfeliciano T, Criado I, Pelak O, Serra-Caetano A, Romero A, Puig N, Remesal A, Torres Canizales J, López-Granados E, Kalina T, Sousa AE, van Zelm M, van der Burg M, van Dongen JJM, Orfao A; EuroFlow PID group

Reference: J Allergy Clin Immunol 2018; 141: 2208-2219.e16

Link: https://www.ncbi.nlm.nih.gov/pubmed/29505809

Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

Lhermitte L, Mejstrikova E, van der Sluijs-Gelling AJ, Grigore GE, Sedek L, Bras AE, Gaipa G, Sobral da Costa E, Novakova M, Sonneveld E, Buracchi C, de Sá Bacelar T, Te Marvelde JG, Trinquand A, Asnafi V, Szczepanski T, Matarraz S, Lopez A, Vidriales B, Bulsa J, Hrusak O, Kalina T, Lecrevisse Q, Martin Ayuso M, Brüggemann M, Verde J, Fernandez P, Burgos L, Paiva B, Pedreira CE, van Dongen JJM, Orfao A, van der Velden VHJ

Reference: Leukemia 2018; 32: 874-881

Link: https://www.ncbi.nlm.nih.gov/pubmed/29089646

Introduction to the diagnosis and classification of monocytic-lineage leukemias by flow cytometry

Matarraz S, Almeida J, Flores-Montero J, Lécrevisse Q, Guerri V, López A, Bárrena S, Van Der Velden VHJ, Te Marvelde JG, Van Dongen JJM, Orfao A

Reference: Cytometry B Clin Cytom 2017; 92: 218-227

Link: https://pubmed.ncbi.nlm.nih.gov/26282340/

Detailed immunophenotyping of B-cell precursors in regenerating bone marrow of acute lymphoblastic leukaemia patients: implications for minimal residual disease detection

Theunissen PMJ, Sedek L, De Haas V, Szczepanski T, Van Der Sluijs A, Mejstrikova E, Nováková M, Kalina T, Lecrevisse Q, Orfao A, Lankester AC, van Dongen JJM, Van Der Velden VHJ on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)

Reference: British Journal of Haematology 2017; 178: 257-266

Link: https://www.ncbi.nlm.nih.gov/pubmed/28419441

Lot-to-lot stability of antibody reagents for flow cytometry

Böttcher S, van der Velden VHJ, Villamor N, Ritgen M, Flores-Montero J, Murua Escobar H, Kalina T, Brüggemann M, Grigore G, Martin-Ayuso M, Lecrevisse Q, Pedreira CE, van Dongen JJM, Orfao A

Reference: Journal of Immunological Methods 2017; doi: 10.1016/j.jim.2017.03.018

Link: https://www.ncbi.nlm.nih.gov/pubmed/28365329

Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples

van der Velden VH, Flores-Montero J, Perez-Andres M, Martin-Ayuso M, Crespo O, Blanco E, Kalina T, Philippé J, Bonroy C, de Bie M, Te Marvelde J, Teodosio C, Corral Mateos A, Kanderová V, van der Burg M, Van Hoof D, van Dongen JJ, Orfao A

Reference: Journal of Immunological Methods 2017; doi: 10.1016/j.jim.2017.03.011

Link: https://www.ncbi.nlm.nih.gov/pubmed/28341440

Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma

Flores-Montero J, Sanoja-Flores L, Paiva B, Puig N, García-Sánchez O, Böttcher S, van der Velden VH, Pérez-Morán JJ, Vidriales MB, García-Sanz R, Jimenez C, González M, Martínez-López J, Corral-Mateos A, Grigore GE, Fluxá R, Pontes R, Caetano J, Sedek L, Del Cañizo MC, Bladé J, Lahuerta JJ, Aguilar C, Bárez A, García-Mateo A, Labrador J, Leoz P, Aguilera-Sanz C, San-Miguel J, Mateos MV, Durie B, van Dongen JJ, Orfao A

Reference: Leukemia 2017; doi: 10.1038/leu.2017.29

Link: https://www.ncbi.nlm.nih.gov/pubmed/28104919

Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia

Theunissen P, Mejstrikova E, Sedek L, van der Sluijs-Gelling AJ, Gaipa G, Bartels M, Sobral da Costa E, Kotrová M, Novakova M, Sonneveld E, Buracchi C, Bonaccorso P, Oliveira E, Te Marvelde JG, Szczepanski T, Lhermitte L, Hrusak O, Lecrevisse Q, Grigore GE, Fronková E, Trka J, Brüggemann M, Orfao A, van Dongen JJ, van der Velden VH on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)

Reference: Blood 2017; 129: 347-357

Link: https://www.ncbi.nlm.nih.gov/pubmed/27903527

Utility of CD54, CD229, and CD319 for the identification of plasma cells in patients with clonal plasma cell diseases

Pojero F, Flores-Montero J, Sanoja L, Pérez JJ, Puig N, Paiva B, Bottcher S, van Dongen JJ, Orfao A on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)

Reference: Cytometry Part B: Clinical Cytometry 2016; 90: 91-100

Link: https://www.ncbi.nlm.nih.gov/pubmed/26130131

Immunophenotype of normal vs. myeloma plasma cells: Toward antibody panel specifications for MRD detection in multiple myeloma

Flores-Montero J, de Tute R, Paiva B, Perez JJ, Böttcher S, Wind H, Sanoja L, Puig N, Lecrevisse Q, Vidriales MB, van Dongen JJ, Orfao A

Reference: Cytometry B Clin Cytom 2016; 90: 61-72

Link: https://pubmed.ncbi.nlm.nih.gov/26100534/

Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality

Bárcena P, Jara-Acevedo M, Tabernero MD, López A, Sánchez ML, García-Montero AC, Muñoz-García N, Vidriales MB, Paiva A, Lecrevisse Q, Lima M, Langerak AW, Böttcher S, van Dongen JJ, Orfao A, Almeida J

Reference: Oncotarget. 2015; 6: 42938-51

Link: https://pubmed.ncbi.nlm.nih.gov/26556869/

Quality Assessment Program for EuroFlow Protocols: Summary results of four-year (2010 - 2013) quality assurance rounds

Tomas Kalina, Juan Flores-Montero, Quentin Lecrevisse, Carlos E. Pedreira, Vincent H.J. van der Velden, Michaela Novakova, Ester Mejstrikova, Ondrej Hrusak, Sebastian Böttcher, Dennis Karsch, Lukasz Sedek, Amelie Trinquand, Nancy Boeckx, Joana Caetano, Vahid Asnafi, Paulo Lucio, Margarida Lima, Ana Helena Santos, Paola Bonaccorso, Alita J. van der Sluijs-Gelling, Anton W. Langerak, Marta Martin-Ayuso, Tomasz Szczepanski, Jacques J.M. van Dongen, Alberto Orfao

Reference: Cytometry Part A 2015; 87A: 145-156

Link: https://www.ncbi.nlm.nih.gov/pubmed/25345353

Overview of clinical flow cytometry data analysis: recent advances and future challenges

Carlos E. Pedreira, Elaine S. Costa, Quentin Lecrevisse, Jacques J.M. van Dongen, Alberto Orfao, on behalf of the EuroFlow Consortium

Reference: Trends in Biotechnology 2013; 31: 415-425

Link: http://www.sciencedirect.com/science/article/pii/S0167779913000942

EuroFlow: Resetting leukemia and lymphoma immunophenotyping. Basis for companion diagnostics and personalized medicine

JJM van Dongen and A Orfao

Reference: Leukemia 2012; 26: 1899-1907

Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437406/

EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes

JJM van Dongen, L Lhermitte, S Böttcher, J Almeida, VHJ van der Velden, J Flores Montero, A Rawstron, V Asnafi, Q Lécrevisse, P Lucio, E Mejstrikova, T Szczepanski, T Kalina, R de Tute, M Brüggemann, L Sedek, M Cullen, AW Langerak, A Mendonça, E Macintyre, M Martin-Ayuso, O Hrusak, MB Vidriales and A Orfao on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006- 018708)

Reference: Leukemia 2012; 26: 1908-1975

Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437410/

Baseline immunophenotypic profile of bone marrow leukemia cells in acute myeloid leukemia with nucleophosmin-1 gene mutation: a EuroFlow study

Matarraz S, Leoz P, Yeguas-Bermejo A, van der Velden, V, Bras AE, Sánchez Galleggo JI, Lecrevisse Q, Ayala-Bueno R, Teodosio C, Criado I, González-González M, Flores-Montero J, Avendaño A, Vidriales MB, Chillón MC, González T, García-Sanz R, Prieto Conde MI, Villamor N, MagnanoL, Colado E, Fernández P, Sonneveld E, Philippé J, Reiterová M, Caballero Berrocal JC, Diaz-Gálvez FJ, Ramos F, Dávila Valls J, Manjón Sánchez R, Solano Tovar J, Calvo X, García Alonso L, Arenillas L, Alonso S, Fonseca A, Quirós Caso C, van Dongen JJM, and Orfao A

Reference: Blood Cancer Journal 2012/13 (1)

Link: https://www.nature.com/articles/s41408-023-00909-4

Flow cytometric immunobead assay for fast and easy detection of PML–RARA fusion proteins for the diagnosis of acute promyelocytic leukemia

EHA Dekking, VHJ van der Velden, R Varro, H Wai, S Böttcher, M Kneba, E Sonneveld, A Koning, N Boeckx, N Van Poecke, P Lucio, A Mendonca, L Sedek, T Szczepanski, T Kalina, V Kanderová, P Hoogeveen, J Flores-Montero, MC Chillón, A Orfao, J Almeida, P Evans, M Cullen, AL Noordijk, PM Vermeulen, MT de Man, EP Dixon, WM Comans-Bitter and JJM van Dongen on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Reference: Leukemia 2012; 26: 1976-1985

Link: https://www.ncbi.nlm.nih.gov/pubmed/22948489

EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

T Kalina, J Flores-Montero, VHJ van der Velden, M Martin-Ayuso, S Böttcher, M Ritgen, J Almeida, L Lhermitte, V Asnafi, A Mendonça, R de Tute, M Cullen, L Sedek, MB Vidriales, JJ Pérez, JG te Marvelde, E Mejstrikova, O Hrusak, T Szczepanski, JJM van Dongen and A Orfao on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Reference: Leukemia 2012; 26: 1986-2010

Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437409/

Harmonization of Light Scatter and Fluorescence Flow Cytometry Profiles Obtained After Staining Peripheral Blood Leucocytes for Cell Surface-Only Versus Intracellular Antigens with the Fix & Perm™ Reagent

Elaine Sobral da Costa, Rodrigo Tosta Peres, Julia Almeida, Quentin Lécrevisse, María Elena Arroyo, Cristina Teodósio, Carlos Eduardo Pedreira, Jacques J. M. van Dongen, and Alberto Orfao on behalf of the EuroFlow Consortium

Reference: Cytometry Part B (Clinical Cytometry) 2010; 78B: 11-20

Link: https://www.ncbi.nlm.nih.gov/pubmed/19575389

Automated pattern-guided principal component analysis vs expert-based immunophenotypic classification of B-cell chronic lymphoproliferative disorders: a step forward in the standardization of clinical immunophenotyping

ES Costa, CE Pedreira, S Barrena, Q Lecrevisse, J Flores, S Quijano, J Almeida, M Del Carmen Garcia-Macias, S Böttcher, JJM van Dongen, A Orfao

Reference: Leukemia 2010; 24: 1927-1933

Link: https://www.ncbi.nlm.nih.gov/pubmed/20844562

Detection of fusion genes at the protein level in leukemia patients via the flow cytometric immunobead assay

E. Dekking, V.H.J, van der Velden, S. Böttcher, M. Brüggemann, E. Sonneveld, A. Koning-Goedheer, N. Boeckx, P. Lucio, L. Sedek, T. Szczepanski, T. Kalina, M. Kovac, P. Evans, P.G. Hoogeveen, J. Flores-Montero, A. Orfao, W.M. Comans-Bitter, F.J.T. Staal, J.J.M. van Dongen on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Reference: Best Practice & Research Clinical Haematology 2010; 23: 333-345

Link: https://www.ncbi.nlm.nih.gov/pubmed/21123134

Flow cytometric immunobead assay for the detection of BCR–ABL fusion proteins in leukemia patients

F Weerkamp, E Dekking, YY Ng, VHJ van der Velden, H Wai, S Böttcher, M Brüggemann, AJ van der Sluijs, A Koning, N Boeckx, N Van Poecke, P Lucio, A Mendonça, L Sedek, T Szczepanski, T Kalina, M Kovac, PG Hoogeveen, J Flores-Montero, A Orfao, E Macintyre, L Lhermitte, R Chen, KAJ Brouwer-De Cock, A van der Linden, ALNoordijk, WM Comans-Bitter, FJT Staal and JJM van Dongen On behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Reference: Leukemia 2009; 23: 1106-1117

Link: https://www.ncbi.nlm.nih.gov/pubmed/19387467

A Probabilistic Approach for the Evaluation of Minimal Residual Disease by Multiparameter Flow Cytometry in Leukemic B-Cell Chronic Lymphoproliferative Disorders

C.E. Pedreira, E.S. Costa, J. Almeida, C. Fernandez, S. Quijano, J. Flores, S. Barrena, Q. Lecrevisse, J.J.M. Van Dongen, A. Orfao, on behalf of the EuroFlow Consortium

Reference: Cytometry Part A. 2008; 73A: 1141-1150

Link: https://www.ncbi.nlm.nih.gov/pubmed/18836994

Generation of Flow Cytometry Data Files with a Potentially Infinite Number of Dimensions

Carlos E. Pedreira, Elaine S. Costa, Susana Barrena, Quentin Lecrevisse, Julia Almeida, Jacques J. M. van Dongen, Alberto Orfao; on behalf of EuroFlow Consortium

Reference: Cytometry Part A. 2008; 73A: 834-846

Link: https://www.ncbi.nlm.nih.gov/pubmed/18629843

EDITORIAL: Infinite multidimensionality

Attila Tárnok

Reference: Cytometry Part A. 2008; 73A: 777-778

Link: https://www.ncbi.nlm.nih.gov/pubmed/18756513