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Standardized EuroFlow Protocols

All EuroFlow Protocols/standard operating procedures (SOPs) are carefully standardized and validated in multi-center trials.

The following protocols are available:

Pre-analytical protocols

  • EuroFlow target MFI values for Rainbow beads. Version 1.10 - November 2023
  • EuroFlow SOP for CSF sample preparation and SST staining. Version 1.0 - May 2024
  • EuroFlow SOP for sample preparation. Version 1.7 - November 2023
  • EuroFlow SOP for bulk lysis in MRD panels. Version 1.3 - June 2018
  • EuroFlow SOP for instrument set-up and compensation for DxFlex. Version 1.2 - July 2023
  • EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto. Version 1.2.1 - October 2019
  • EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 1.8 - July 2019
  • EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.2 - September 2024
  • EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 - October 2019
  • EuroFlow guidelines for BM sample collection and transport for MM MRD measurements. Version 1.0 - April 2017

Analytical protocols

  • Composition and technical info of EuroFlow panels for PID. Version 1.2 - September 2022
  • Composition and technical info of EuroFlow panels for hematological malignancies. Version 1.11 - November 2023
  • List of reference and alternative antibodies for hematological malignancies panels. Version 1.13 - November 2023
  • Uniform coding of EuroFlow panels. Version 1.1 - March 2019

Data analysis

  • Analysis template BCP-ALL MRD panel (Verbeek et al., 2021)

Downloads for the BD Lyric

  • Reagent files (zip) (download and extract)
  • Assay files (zip) (download and extract)
  • Label file (xml)

Reference values per age group

  • Distribution major populations of blood B-cells and plasma cells children (EuroFlow IgH-isotypes tube). Version 1.1 - July 2024
  • Distribution major populations of blood TCD4 cells in healthy donors grouped by age (EuroFlow IMM TCD tube), version 1.1 - July 2024
  • Reference values for PB leukocyte populations (EuroFlow LST) version 1.1 - July 2024
  • Reference values for PB leukocyte populations in percentages (EuroFlow PIDOT). Version 1.2 - December 2024
  • Reference values for PB leukocyte populations in absolute numbers of cells (EuroFlow PIDOT). Version 1.1 - July 2024

To download the protocols, please register and log in via this link.

Educational videos

BulkLysis

Instrument Setup & Compensation

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