Standardized EuroFlow Protocols
All EuroFlow Protocols/standard operating procedures (SOPs) are carefully standardized and validated in multi-center trials.
The following protocols are available:
Pre-analytical protocols
- EuroFlow target MFI values for Rainbow beads. Version 1.10 - November 2023
- EuroFlow SOP for CSF sample preparation and SST staining. Version 1.0 - May 2024
- EuroFlow SOP for sample preparation. Version 1.7 - November 2023
- EuroFlow SOP for bulk lysis in MRD panels. Version 1.3 - June 2018
- EuroFlow SOP for instrument set-up and compensation for DxFlex. Version 1.2 - July 2023
- EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto. Version 1.2.1 - October 2019
- EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 1.8 - July 2019
- EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.2 - September 2024
- EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 - October 2019
- EuroFlow guidelines for BM sample collection and transport for MM MRD measurements. Version 1.0 - April 2017
Analytical protocols
- Composition and technical info of EuroFlow panels for PID. Version 1.2 - September 2022
- Composition and technical info of EuroFlow panels for hematological malignancies. Version 1.11 - November 2023
- List of reference and alternative antibodies for hematological malignancies panels. Version 1.13 - November 2023
- Uniform coding of EuroFlow panels. Version 1.1 - March 2019
Data analysis
- Analysis template BCP-ALL MRD panel (Verbeek et al., 2021)
Downloads for the BD Lyric
- Reagent files (zip) (download and extract)
- Assay files (zip) (download and extract)
- Label file (xml)
Reference values per age group
- Distribution major populations of blood B-cells and plasma cells children (EuroFlow IgH-isotypes tube). Version 1.1 - July 2024
- Distribution major populations of blood TCD4 cells in healthy donors grouped by age (EuroFlow IMM TCD tube), version 1.1 - July 2024
- Reference values for PB leukocyte populations (EuroFlow LST) version 1.1 - July 2024
- Reference values for PB leukocyte populations in percentages (EuroFlow PIDOT). Version 1.2 - December 2024
- Reference values for PB leukocyte populations in absolute numbers of cells (EuroFlow PIDOT). Version 1.1 - July 2024
To download the protocols, please register and log in via this link.
Educational videos
BulkLysis
Instrument Setup & Compensation
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